I8443-42F
Interleukin 23 (IL-23)
Size
£819.00
- SKU:
- I8443-42F
- Application:
- WB
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Sheep
- Immunogen:
- Recombinant protein corresponding to Arg23-Pro193 from canine IL-23 p19 expressed in E. coli.
- Format:
- Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- IL-23 p19 is an 18-20kD (estimated) member of the IL-6 superfamily of molecules. It is disulfide-bonded to p40 to form IL-23. IL-23 is secreted by immune-related cell types such as keratinocytes, dendritic cells, macrophages, microglia and monocytes, often following TLR stimulation. It appears to drive Th17 cell development by inhibiting T-bet and FoxP3 production, and to reduce IL-12-mediated IFN-g production by CD4+, CD8+ and NK cells. Based on human, mature canine IL-23 p19 is 171aa in length. It is a a-helical molecule that utilizes Cys77 to form an interchain disulfide bond with IL-12 p40. IL-23 p19 does not appear to be released unless dimerized to p40. There are no potential N-linked glycosylation sites on p19. Mature canine IL-23 p19 aa23-193 shares 88aa identity with human IL-23 p19. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.5ug/ml with human mature dendritic cells (LPS activated), CLL‑1390 canine leukocytic round cell neoplasia cell line, and THP‑1 human acute monocytic leukemia cell line. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice