I8443-42E
Interleukin 23 (IL-23)
Size
£806.00
- SKU:
- I8443-42E
- Application:
- WB
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Sheep
- Immunogen:
- Recombinant corresponding to Arg26-Arg185 from feline IL-23 p19 expressed in E. coli (ABB01676).
- Format:
- Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with 500ulsterile PBS.
- Extra Details:
- IL-23 p19 is an 18-20kD (estimated) member of the IL-6 superfamily of molecules. It is disulfide-bonded to p40 to form IL-23. IL-23 is secreted by immune-related cell types such as keratinocytes, dendritic cells, macrophages, microglia and monocytes, often following TLR stimulation. It appears to drive Th17 cell development by inhibiting T-bet and FoxP3 production, and to reduce IL-12-mediated IFN-g production by CD4+, CD8+ and NK cells. Based on human, mature feline IL-23 p19 is 160aa in length. It is a a-helical molecule that utilizes Cys80 to form an interchain disulfide bond with IL-12 p40. IL-23 p19 does not appear to be released unless dimerized to p40. There are no potential N-linked glycosylation sites on p19. Mature feline IL-23 p19 aa26-185 shares 87aa identity with human IL-23 p19. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice