I8443-18T
Interleukin 22 (IL-22, IL-10 Related T cell-derived Inducible Factor, IL-TIF)
Size
£882.00
- SKU:
- I8443-18T
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant human IL-22 (Ala34-Ile179) expressed in E. coli (Accession #Q9GZX6)
- Clone:
- 11D67
- Format:
- Supplied as a powder lyophilized from a solution containing PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
- Extra Details:
- Interleukin 22 (IL-22), also called IL-10-related T cell-derived inducible factor or IL-TIF, is a secreted cytokine that contributes to the inflammatory response in vivo. IL-22 is expressed in T cells and mast cells in response to IL-9 or lipopolysaccharide. IL-22 monomers bind to a class II cytokine receptor that is a heterodimer of CRF2-9 (IL-22 Ra1) and CRF2-4 (IL-10 Rb). Human IL-22 is a 179aa protein with a 33aa signal peptide, three N-glycosylation sites and two intramolecular disulfide bonds; the mature protein shows 81% and 77% aa identity with mouse and rat IL-22, respectively. Applications: Suitable for use in ELISA and Neutralization. Other applications not tested. Recommended Dilution: Neutralization: Measured by its ability to neutralize IL-22 induced IL-10 secretion in the COLO 205 human colorectal adenocarcinoma cell line. Nagalakshmi, M.L. et al. (2004) Intl. Immunopharmacol. 4:679. The Neutralization Dose (ND50) is typically 1-5ug/ml in the presence of 1ng/ml recombinant human IL-22. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice