I8443-17A
Interleukin 22 (IL-22, IL-10 Related T cell-derived Inducible Factor, IL-TIF)
Size
£889.00
- SKU:
- I8443-17A
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Ala34-Ile179 from human IL-22 epressed in E. coli.
- Clone:
- 142906
- Format:
- Supplied as a powder lyophilized from a solution containing PBS and trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Interleukin22 (IL22), also known as IL10related T cellderived inducible factor (ILTIF) was initially identified as a gene induced by IL9 in mouse T cells and mast cells. Human IL22 cDNA encodes a 179 aa residue protein with a putative 33 aa signal peptide that is cleaved to generate a 147 aa mature protein that shares approximately 79% and 22% aa sequence identity with mouse IL22 and human IL10, respectively. The human IL22 gene is localized to chromosome 12q15. Although it exists as a single copy gene in human and in many mouse strains, the mouse IL22 gene is duplicated in some mouse strains including C57B1/6, FVB and 129. The two mouse genes designated ILTIF-alpha and ILTIF-beta, share greater than 98% sequence homology in their coding region. IL22 has been shown to activate STAT1 and STAT3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL22 is produced by normal T cells upon antiCD3 stimulation in humans. Mouse IL22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL22 may be involved in inflammatory responses. The functional IL22 receptor complex consists of two receptor subunits, IL22R (previously an orphan receptor named CRF29) and IL10 R-beta (previously known as CRF24), belonging to the class II cytokine receptor family. Applications: Suitable for use in ELISA and Western Blot. Other applications have not been tested. Recommended Dilutions: ELISA: 2-8ug/ml when used as capture antibody and paired with I8443-17B (used at 0.5-2ug/ml) as detection antibody. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice