I8442-44
Interleukin 32 alpha (IL-32a, Interleukin-32 Precursor, Natural Killer Cells Protein 4, NK4, TAIF, TAIFa, Tumor Necrosis Factor alpha-inducing Factor)
Size
£761.00
- SKU:
- I8442-44
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Recombinant protein corresponding to Phe3-Lys131 from human IL‑32, expressed in E. coli.
- Clone:
- 11n107
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- IL-32a is the shortest and most abundant of four potential splice variants of the proinflammatory cytokine IL-32 (previously called NK4) with a predicted unmodified size of 15kD. Potential modifications include myristoylation and N-glycosylation. Transfected IL-32a was more likely to be cell-associated as compared to IL-32b, suggesting an intracellular function. IL-32 is induced by mitogens in peripheral lymphocytes, by IFN-g in epithelial cells, or by IL-12 with IL-18 in NK cells and in turn Induces cytokine expression. No ortholog has been found in mouse. Applications: Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml Immunocytochemistry: 8-25ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice