I8439-28D
Interleukin 17F (IL-17F)
Size
£825.00
- SKU:
- I8439-28D
- Purity:
- Purified by Protein A affinity chromatography from hybridoma culture supernatant
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Arg31-Gln163 from human Interleukin 17F expressed in E. coli.
- Clone:
- 8H115
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Interleukin-17F (IL-17F) is a disulfide-linked dimer belonging to the IL-17 family. IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions. Applications: Suitable for use in Direct ELISA, Immunocytochemistry and Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry (Intracellular): 2.5ug/10e6 cells using human peripheral blood mononuclear cells treated with PMA and calcium ionomycin, fixed with paraformaldehyde and permeabilized with saponin. Immunocytochemistry: 8-25ug/ml using immersion fixed human TH17 cells Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice