I8439-21D
Interleukin 17 Receptor D (IL-17RD)
Size
£812.00
- SKU:
- I8439-21D
- Purity:
- Purified by Protein G affinity chromatography from cell culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Recombinant protein corresponding to Gly28-Arg299 from mouse IL-17 Receptor D, expressed in NS0 cells (Q8JZL1)
- Clone:
- 8H170
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Interleukine-17 receptor D (IL-17 RD) also known as Sef (similar expression to FGFs), is a type I transmembrane IL-17R-related protein that can bind to FGF receptors 1 and 2 and antagonize ERK signaling by preventing translocation to the nucleus. The full-length 738aa mouse Sef-a is a 120kD cell surface glycoprotein expressed in most tissues (except thyroid). Mouse IL-17 RD shows 90% aa identity with human IL-17 RD in the extracellular/lumenal region. Applications: Suitable for use in ELISA, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: 8-25ug/ml on perfusion fixed frozen sections of mouse liver and mouse thymus and immersion fixed frozen sections of mouse lung. Flow Cytometry: 0.25ug/10e6 cells in in HEK293 human cell line transfected with mouse IL-17 RD/SEF Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice