I8439-20P
Interleukin 17BR (IL-17BR, IL-17B Receptor)
Size
£1,004.00
- SKU:
- I8439-20P
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to the extracellular domain (Arg18-Gly289) of human IL-17BR, expressed in NSO cells.
- Clone:
- 8B27
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
- Extra Details:
- Interleukin 17B receptor (IL-17B R) is the second identified receptor for the IL-17 family of cytokines. IL-17B R binds strongly to IL-17E and binds weakly to IL-17B, but does not bind IL-17, IL-17C or IL-17F. It is a transmembrane protein expressed in a wide variety of tissues. Applications: Suitable for use in ELISA and Western Blot. Other applications have not been tested. Recommended Dilutions: ELISA (Capture): 2-8ug/ml using I8439-20L (Interleukin 17B (Biotin)) as the detection antibody. Western Blot: 1-2ug/ml Using a colorimetric detection system, the detection limit is ~25ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5-50 fold. Optimal dilutions to be determined by the researcher. Hybridoma: NSO myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice