I8438-05
Interleukin 16 (LCF, prIL-16, FLJ16806, FLJ42735, FLJ44234, HST19289 )
Size
£614.00
- SKU:
- I8438-05
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to 129aa from human IL-16 expressed in E. coli.
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
- Extra Details:
- Interleukin-16, also known as lymphocyte chemoattractant factor (LCF), is a chemotactic cytokine that stimulates a migratory response in CD4+ lymphocytes, monocytes and eosinophils through binding to the CD4 receptor. IL-16 induces human T-lymphocyte expression of interleukin 2 receptor and upregurates major histocompatibility complex molecules on resting human T lymphocyte. It is able to render CD4+ T cells unresponsive to TCR/CD3 stimuli. Moreover, IL-16 inhibits the replication of T cell and monocyte tropic HIV strains in vitro. Applications: Suitable for use in Neutralization, ELISA and Western Blot. Other applications not tseted. Recommended Dilutions: Neutralization: 0.12-0.15ug/ml will yield one-half maximal inhibition [ND50] of the biological activity of hIL-16 (4.2ng/ml). ELISA: ≥0.5ug/ml using 100ul/well antibody solution. Allows the detection of 0.2-0.4ng/well of recombinant hIL-16. Western Blot: 0.1-0.2ug/ml The dectection limit is 1.5-3.0ng/lane, under either reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice