I8435-10
Interleukin 13, Recombinant, Human (IL-13)
Size
£332.00
- SKU:
- I8435-10
- Molecular Weight:
- 12.5
- Purity:
- ≥98% (SDS-PAGE and HPLC). Endotoxin: ≤0.1ng/ug (1EU/ug).
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Format:
- Supplied as a lyophilized powder with no additives. Reconstituted with sterile ddH2O to a concentration of 0.1-1.0mg/ml. Do not vortex.
- Extra Details:
- IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and also by mast cells and NK cells. Targeted deletion of IL-13 in mice resulted in impaired Th2 cell development and indicated an important role for IL-13 in the expulsion of gastrointestinal parasites. IL-13 exerts anti-inflammatory effects on monocytes and macrophages and it inhibits the expression of inflammatory cytokines such as IL-1b, TNF-a, IL-6 and IL-8. IL-13 has also been shown to enhance B cell proliferation and to induce isotype switching resulting in increased production of IgE. Blocking of IL-13 activity inhibits the pathophysiology of asthma. Human and murine IL-13 is cross-species reactive. Recombinant human IL-13 is a 12.5kD protein consisting of 114 amino acid residues, expressed in E. coli. Biological Activity: The ED50 as determined by the dose-dependent proliferation of TF-1 cells was ≤1ng/ml, corresponding to a specific activity of ≥1x10e6u/mg. Amino Acid Sequence: SPGPVPPSTA LRELIEELVN ITQNQKAPLC NGSMVWSINL TAGMYCAALE SLINVSGCSA IEKTQRMLSG FCPHKVSAGQ FSSLHVRDTK IEVAQFVKDL LLHLKKLFRE GRFN Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Ambient