I8435-09
Interleukin 13 (IL-13)
Size
£442.00
- SKU:
- I8435-09
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to mouse Interleukin 13, expressed in E.coli.
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile ddH2O to 0.1-1mg/ml.
- Extra Details:
- Applications: Suitable for use in ELISA, Western Blot and Neutralization. Other applications have not been tested. Recommended Dilutions: ELISA (Sandwich): 0.5-2ug/ml (using100ul/well antibody solution). In conjunction with biotinylated anti-mouse IL-13 (I8435-06), detects 0.2-0.4ng/well of recombinant mouse IL-13. Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mouse IL-13 is 1.5-3.0ng/lane, under either reducing or non-reducing conditions. Neutralization: 0.08-0.1ug/ml will yield one-half maximal inhibition [ND50] of the biological activity of mouse IL-13 (10ng/ml). Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice