I8434-50B
Interleukin 12 Receptor, beta 2 (IL-12Rb2) (PE)
Size
£806.00
- SKU:
- I8434-50B
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant
- Storage Conditions:
- 4°C Do not freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Cys28-Asn622 from human Interleukin 12 Receptor beta 2 extracellular domain, expressed in NS0 cells.
- Clone:
- 9H1
- Format:
- Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
- Extra Details:
- The biological activities of IL-12 are mediated through the high-affinity receptor complex composed of the IL-12 receptor b1 (IL-12 Rb1) and IL-12 receptor b2 (IL-12 Rb2) subunits. IL-12 Rb2 is a 130kD protein that interacts with p35 and is associated with Jak2. The expression of IL-12 Rb2 is absent on naïve T cells. Activation of T cells via TCR upregulates IL-12 Rb2 expression on human Th1, but not Th2 cells. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul labels 1x10e6 cells. Human CD4+ peripheral blood mononuclear cells (PBMCs) treated with mouse anti-human CD3e monoclonal antibody, Recombinant human IL-12 and Recombinant human IL-2 to induce Th1 cell development, followed by restimulation with PMA and calcium Ionomycin. Optimal dilutions to be determined by the researcher. Hybridoma: NS0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice