I8432-27A

Interleukin 10 (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF)

Size

£863.00

SKU:: I8432-27A
Purity:: Purified by Protein A affinity chromatography from ascites. Endotoxin: ≤0.1EU/mg
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Ser19-Asn178 of human Interleukin 10 expressed in E. coli.
Clone:: 25209
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: Applications: Suitable for use in ELISA, Western Blot and Neutralization. Other applications not tested. Recommended Dilutions: Neutralization (ND50): 0.005-0.03ug/ml in the presence of 5ng/ml recombinant human IL IL--10. Measured by its ability to neutralize IL--10 induced proliferation in the MC/9-2 mouse mast cell line. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.