I8430

Interleukin 8, Recombinant, Human, 72aa (IL-8)

Size

£338.00

SKU:: I8430
Molecular Weight:: 8.4
Purity:: ≥ 98% by SDS-PAGE and HPLC Endotoxin:≤ 0.1ng/ug
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder without additives. Reconstitute with sterile dH2O to a concentration of 0.1-1mg/ml. This solution can then be diluted into other aqueous solutions.
Extra Details:: It promotes neutrophil chemotaxis and degranulation. The 72 amino acid IL-8 is the predominant form secreted by monocytes and lymphocytes. Recombinant human interleukin-8 (IL-8) is an 8.4kD protein containing 72 amino acid residues, expressed in E. coli. AA Sequence: SAKELRCQCI KTYSKPFHPK FIKELRVIES GPHCANTEII VKLSDGRELC LDPKENWVQR VVEKFLKRAE NS Biological Activity: Human IL-8 produces chemotaxis over a wide concentration range in an assay using human peripheral blood neutrophils. Significant chemotaxis was achieved using a concentration of 10-100ng/ml. Responding Cells (partial list): Neutrophils, T-Lymphocytes Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Stable for at least 6 months at -20°C. Reconstitute with sterile deionized water to a concentration ≥100ug/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.