Interleukin 8 (IL-8)

Size

£629.00

SKU:: I8430-27
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to human IL-8, expressed in E. coli.
Format:: Supplied as a lyophilized powder in PBS. Reconstitute with 100ul sterile ddH2O.
Extra Details:: IL-8, also known as CXCL8, is produced by a range of cells including stimulated monocytes, mast cells, endothelial cells, keratinocytes and fibroblasts. Human IL-8 promotes neutrophil chemotaxis and degranulation. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 0.5-2ug/ml using 100ul/well. The detection limit is 0.2-0.4ng/well of recombinant human IL-8 Western Blot: 0.1-0.2ug/ml. The detection limit for recombinant human IL-8 is 1.5-3ng/lane under either reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.