I8430-15
Interleukin 8 (IL-8)
Size
£435.00
- SKU:
- I8430-15
- Purity:
- Purified by Protein G affinity chromatography from cell culture.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to human Interleukin 8 expressed in E. coli.
- Clone:
- 8.PP.6
- Format:
- Supplied as a lyophilized powder from PBS. Reconstitute with 500ul sterile ddH2O.
- Extra Details:
- Applications: Suitable for use in ELISA and Western Blot. Other applications have not tested. Recommended Dilutions: ELISA (Sandwich): 2-4ug/ml using 100ul/well antibody solution In conjunction with biotinylated anti-human Interleukin 8 (I8430-03) as a detection antibody, allows the detection of at least 1000pg/ml of recombinant human Interleukin 8. Western Blot: 0.2-0.4ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human Interleukin 8 is 2-4ng/lane under non-reducing conditions. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-65D: IgG, H&L (AP) Pab Rb xMo I1904-65J: IgG, H&L, X-Adsorbed (AP) Pab Rb xMo I1904-65P: IgG (AP) Pab Rb xMo Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Ambient