I8430-02M
Interleukin 8 (IL-8)
Size
£1,042.00
- SKU:
- I8430-02M
- Purity:
- Purified by Protein G affinity chromatography purified from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- E. coli-derived recombinant protein corresponding to Ala23-Pro101 from canine CXCL8.
- Clone:
- 7H60
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
- Extra Details:
- CXCL8, also known as IL-8, is a chemoattractant that recruits neutrophils to sites of inflammation, activates neutrophil functions and promotes angiogenesis. CXCL8 production is rapidly induced by pro-inflammatory signals in many different cell types. The biological activites of CXCL8 are mediated by chemokine receptors CXCR1 and CXCR2. Feline CXCL8 is translated as a 101 aa precursor protein with a 22 aa signal peptide. It shares 61% and 76% aa sequence identity with human and canine CXCL8, respectively. Applications: Suitable for use in Neutralization and ELISA. Other applications not tested. Recommended Dilutions: ELISA: 2-8ug/ml. Neutralization: Measured by its ability to neutralize CXCL8/IL‑8-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR2. The Neutralization Dose (ND50) is typically 3-12ug/ml in the presence of 10ng/ml recombinant Canine CXCL8/IL‑8. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice