I8430-02E

Interleukin 8 (IL-8, 3-10C, AMCF-I, b-ENAP, CXCL8, C-X-C motif chemokine 8, Emoctakin, GCP1, GCP-1, Granulocyte chemotactic protein 1, K60, LECT, LUCT, LYNAP, MDNCF, MONAP, Monocyte-derived neutrophil-activating peptide, Monocyte-derived neutrophil chemotactic factor, NAF, NAP1, NAP-1, Neutrophil-activating protein 1, Protein 3-10C, SCYB8, T-cell chemotactic factor, TSG-1)

Size

£576.00

SKU:: I8430-02E
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human IL-8.
Clone:: 3H209
Format:: Supplied as a lyophilized powder in 0.01M PBS, pH 7.0. Reconstitute with sterile ddH2O to ~1mg/ml.
Extra Details:: IL-8, also known as CXCL8, is produced by a range of cells including stimulated monocytes, mast cells, endothelial cells, keratinocytes and fibroblasts. Human IL-8 promotes neutrophil chemotaxis and degranulation. Applications: Suitable for use in ELISA, Neutralization and Western Blot. Other applications not tested. Recommended Dilution: Neutralizing: In chemotaxis assay, the antibody inhibited 97.8% of the chemotactic activity of IL-8 on RB/293 cell using 5ug/ml of I8430-02E and 10ng/ml of human IL-8 in assay. I8430-02E inhibited the chemotactic effect of human IL-8, had no inhibitory effect to MIP 1-bata and RANTES in neutrophil chemotaxis assay, indicating the antibody’s ability to neutralize IL-8 activity. I8430-02E has shown neutralizing activity on calcium ion changes in human granulocytes using FACS. Western Blot: 0.02-0.1ug/ml allows visualization of 100ng/lane of human IL-8. Optimal dilutions to be determined by researcher. Matched Pair: I8430-02A: Dectection I8430-02E: Capture Hybridoma: Sp2/0-Ag14 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice