I8430-02B

Interleukin 8 (IL-8)

Size

£486.00

SKU:: I8430-02B
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified recombinant human IL-8
Clone:: 3H206 (I8-61)
Format:: Supplied as a lyophilized powder from PBS, pH 7.0. Reconstitute with 100ul sterile ddH2O.
Extra Details:: Applications: Suitable for use in ELISA, Neutralizing and Western Blot. Other applications not tested. Recommended Dilution: Neutralizing: In chemotaxis assay, the antibody inhibited 97% of the chemotactic activity of IL-8 on RB/293 cell using 5ug/ml of I8430-02B and 10ng/ml of human IL-8 in assay. I8430-02B inhibited the chemotactic effect of human IL-8, had no inhibitory effect to MIP 1-bata and RANTES in neutrophil chemotaxis assay, indicating the antibody’s ability to neutralize IL-8 activity. I8430-02B has shown neutralizing activity on calcium ion changes in human granulocytes using FACS. Western Blot: 0.02-0.1ug/ml allows visualization of 100ng/lane of human IL-8. Optimal dilutions to be determined by researcher. Myeloma: P3X63Ag8.653 Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.