I8430-02A
Interleukin 8 (IL-8, 3-10C, AMCF-I, b-ENAP, CXCL8, C-X-C Motif Chemokine 8, Emoctakin, GCP1, GCP-1, Granulocyte Chemotactic Protein 1, K60, LECT, LUCT, LYNAP, MDNCF, MONAP, Monocyte-derived Neutrophil-activating Peptide, Monocyte-derived Neutrophil Chemotactic Factor, NAF, NAP1, NAP-1, Neutrophil-activating Protein 1, Protein 3-10C, SCYB8, T-cell Chemotactic Factor, TSG-1)
Size
£294.00
- SKU:
- I8430-02A
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified recombinant human IL-8.
- Clone:
- 3H205
- Format:
- Supplied as a liquid in PBS, pH 7.0.
- Extra Details:
- IL-8, also known as CXCL8, is produced by a range of cells including stimulated monocytes, mast cells, endothelial cells, keratinocytes and fibroblasts. Human IL-8 promotes neutrophil chemotaxis and degranulation. Applications: Suitable for use in ELISA, Neutralizing, Immunohisotchemistry and Western Blot. Other applications not tested. Recommended Dilutions: Neutralizing: 5ug/ml. In chemotaxis assay, the antibody inhibited 98.4% of the chemotactic activity of IL-8 on RB/293 cell using 5ug/ml of I8430-02A and 10ng/ml of human IL-8 in assay. I8430-02A inhibited the chemotactic effect of human IL-8, had no inhibitory effect to MIP 1-bata and RANTES in neutrophil chemotaxis assay, indicating the antibody’s ability to neutralize IL-8 activity. I8430-02A has shown neutralizing activity on calcium ion changes in human granulocytes using FACS. Western Blot: 0.02-0.1ug/ml allows visualization of 100ng/lane of human IL-8. Optimal dilutions to be determined by researcher. Recommended Matched Pair: I8430-02A (Dectection): Interleukin 8 (IL-8) I8430-02E (Capture): Interleukin 8 (IL-8) Hybridoma: P3X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice