I8429-27M1

Interleukin 7 Receptor alpha (Interleukin-7 Receptor Subunit alpha, IL-7 Receptor Subunit alpha, IL-7R Subunit alpha, IL-7R-alpha, IL7RA, IL-7RA, IL7R, CD127, CDw127, ILRA)

Size

£557.00

SKU:: I8429-27M1
Application:: Flow Cytometry
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant correspponding to human CD127.
Clone:: 11K10
Format:: Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 0.5mg/ml gentamicin sulfate.
Extra Details:: CD127 (IL-7R alpha) is the specific receptor component for the cytokine Interleukin -7 (IL-7). It is found on a wide variety of hematopoietic cell types including B cell precursors, and the majority of T cells. Its expression levels are decreased on T cells following activation. CD127 can dimerize with CD132 (IL-2R gamma) to form a high affinity IL-7 receptor (1). CD127 engagement is necessary for T cell development in humans (2). Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. 5x10e5 human Nalm-6 cells were washed incubated 45 minutes on ice with 80ul of I8429-27M1 at 10ug/ml. 2. Cells were washed twice and incubated with IgG (FITC) Goat-anti-Mouse . 3. The cells were washed twice, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.71 log10 fluorescent units when compared to isotype control Mouse IgG1 antibody. Binding was blocked when reagent was pre-incubated with a 10-fold excess of recombinant CD127-muIg (cat# I8429-27N). Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice