Interleukin 7 (IL-7) (Biotin)

Size

£512.00

SKU:: I8429-26
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to mouse Interleukin 7 expressed in E. coli.
Format:: Supplied as a lyophilized powder from PBS, pH 7.2. Labeled with Biotin. Reconstitute with sterile PBS, 0.1% BSA.
Extra Details:: Applications: Suitable for use in ELISA and Western Blot. Other applications have not been tested. Recommended Dilutions: ELISA (Direct): 1ug/ml (using 100ul/well) Used in conjunction with compatible secondary reagents the detection limit is 0.2-0.4ng/lane of recombinant mouse IL-7. ELISA (Sandwich): 0.25-1ug/ml (using 100ul/well) Used in conjunction with I8429-25A as capture, detection limit is 0.2-0.4ng/lane of recombinant mouse IL-7. Western Blot: 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit is 1.5-3ng/lane of recombinant mouse IL-7, under either reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.