Interleukin 7 (IL-7) (Biotin)

Size

£541.00

SKU:: I8429-11D
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to human IL-7, expressed in E. coli.
Format:: Supplied as a lyophilized powder from PBS, pH 7.2. Labeled with Biotin. Reconstitute with 1ml sterile PBS, 0.1% BSA.
Extra Details:: Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: using sandwich ELISA (100ul/well antibody solution) a concentration of 0.25-1ug/ml is required. In conjunction with I8429-15P as a capture antibody, allows the detection of at least 0.2-0.4ng/well of recombinant Human IL-7. Western Blot: 0.1-0.2ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant hIL-7 is 1.5-3.0ng/lane, under either reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.