I8428

Interleukin 6, Recombinant, Human (IL-6)

Size

£338.00

SKU:: I8428
Molecular Weight:: 20.9
Purity:: ≥98% by SDS-PAGE and HPLC. Endotoxin: ≤0.1ng/ug.
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Reconstitute with sterile ddH2O to a concentration of 0.1-0.5mg/ml. Gently shake the solution for about 10 minutes for better solubility. Do not vortex.
Extra Details:: IL-6 is a pleiotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses. Produced by T cells, monocytes, fibroblasts, endothelial cells and keratinocytes, IL-6 has diverse biological functions. It stimulates B-cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. IL-6 signals through the IL-6 receptor system that consists of two chains, IL-6Ra and gp130. Murine IL-6 is inactive on human cells, while both human and murine are equally active on murine cells. Recombinant human IL-6 is a 20.9kD protein containing 184aa residues. Biological Activity: Fully biologically active when compared to standards. The ED50 was determined by the dose dependent stimulation of the proliferation of IL-6 dependent murine 7TD1 cells is ≤0.1ng/ml, corresponding to a specific activity of ≥1x10e7units/mg. Amino Acid Sequence: PVPPGEDSKD VAAPHRQPLT SSERIDKQIR YILDGISALR KETCNKSNMC ESSKEALAEN NLNLPKMAEK DGCFQSGFNE ETCLVKIITG LLEFEVYLEY LQNRFESSEE QARAVQMSTK VLIQFLQKKA KNLDAITTPD PTTNASLLTK LQAQNQWLQD MTTHLILRSF KEFLQSSLRA LRQM Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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