I8428-39L1
Interleukin 6 Receptor (CD126, IL-6R) (APC)
Size
£800.00
- SKU:
- I8428-39L1
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- 4°C Do not freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Leu20-Asp339 from human IL-6R, expressed in Sf21 cells.
- Clone:
- 8H112
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
- Extra Details:
- The multifunctional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80kD component receptor that binds IL-6 with low affinity (IL-6 R) and a signal- transducing component of 130kD (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex. Both components of the receptor complex, IL-6 R and gp130 have been cloned, sequenced, and expressed. A soluble form of the IL-6 R has been found in the urine of healthy adult humans . This soluble receptor apparently arises from proteolytic cleavage of membrane-bound IL-6 R. No naturally-occurring mRNA encoding a truncated form of the IL-6 R has been reported. Soluble forms of human and murine IL-6 Rs have been constructed, however, by insertion of termination codons into the regions of the IL-6 R cDNAs encoding the external portions of the receptors prior to the transmembrane domains. These soluble receptors have been expressed in COS-7 and CHO cells and have been shown to bind to IL-6 in solution and to augment the activity of IL-6 as a result of the binding of the IL-6/IL-6 R complex to membrane-bound gp130. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: Neat. Use 10ul to label 1x10e6 cells. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice