I8428-28

Interleukin 6 (IL-6)

Size

£863.00

SKU:: I8428-28
Purity:: Purified by Protein G affinity chromatography from cell culture supernatant. Endotoxin: ≤0.1EU/ug (LAL)
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human IL-6, expressed in E. coli.
Clone:: 6708
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: Interleukin 6 (IL-6) is a cytokine produced primarily by stimulated monocytes, fibroblasts, and epithelial cells. It has a wide variety of biological functions including activating cell surface signaling assemblies composed of IL6, IL6RA, and the shared signaling receptor gp130. The cytokine plays an essential role in the final differentiation of b-cells into Ig secreting cells and induces myeloma, plasmacytoma growth, and nerve cell differentiation. In addition, IL-6 is an important regulator of acute phase reactions. Ota et al. (2001) presented evidence that variations in IL-6 may be related to osteoporosis. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, and Neutralizaion. Other applications not tested. Recommended Dilutions: ELISA (Capture): 2-8ug/ml ELISA (Detection): 0.1-0.4ug/ml Western Blot: 1ug/ml using recombinant human IL-6 Immunohistochemistry: 8-25ug/ml using fixed frozen sections of hyperplastic human skin Neutralization: The ND50 is typically 8-80ng/ml in the presence of 2.5ng/ml recombinant human IL-6. Optimal dilution to be determined by the researcher. Recommeded Pair: Capture: I8428-28 Detection: I8428-29 Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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