I8428-23T
Interleukin 6 (IL-6)
Size
£524.00
- SKU:
- I8428-23T
- Purity:
- Purified by Ion Exchange chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to full length human IL-6 expressed in E. coli.
- Clone:
- 3H2542 (B-E8)
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide
- Extra Details:
- IL-6 is a cytokine with a wide variety of biological functions. It plays an essential role in the final differentiation of B-cells into Ig-secreting cells. It induces myeloma and plasmacytoma growth, nerve cells differentiation in hepatocytes, and acute phase reactants. Applications: Suitable for use in Immunohistochemistry, ELISA and Neutralization Assays. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: Frozen sections. The epitope recognized is sensitive to formaldehyde fixation amd tissue processing. Using acetone fixation is recommended. Neutralizing Assay: 20pg neutralizes activity of 1U of IL-6. Note: Removal of azide is required. Optimal working dilutions to be determined by researcher. Recommended Secondary Reagents: I1904-65W: IgG, F(ab’)2 (HRP) Pab Rb xMo I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab Rb xMo Recommended Negative Controls: I1904-78R: IgG1 Murine Negative Control Hybridoma: Spleen cells from BALB/c mice were fused with cells of the mouse X63 Ag8.653 myeloma cell line. Affinity: 0.5nM Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice