I8428-07C1
Interleukin 6 (IL-6) (FITC)
Size
£986.00
- SKU:
- I8428-07C1
- Purity:
- Purified by Protein A/G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified recombinant human IL-6
- Clone:
- 5J42
- Format:
- Supplied as a liquid in PBS, pH 7.2. Carrier and preservative free. Labeled with Fluorescein isothiocyanate (FITC).
- Extra Details:
- Interleukin-6 (IL-6) is a pleotropic cytokine that plays an important role in host defense by regulating immune and inflammatory responses. IL-6 is produced by T cells, monocytes, fibroblasts, endothelial cells and keratinocytes. It has diverse biological functions. It stimulated B cell differentiation and antibody production. It synergizes with IL-3 in megakaryocyte development and platelet production. It induces expression of hepatic acute phase proteins. It regulated bone metabolism. IL-6 signals through the IL-6 receptor system that consists of two chains: IL-6Ra and gp130. Applications: Suitable for use in Flow Cytometry, FLISA and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 1-10ul stains 10e6 human peripheral blood mononuclear cells stimulated with LPS. Successfully detects intracellular IL-6 levels. FLISA: detection Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice