I8428-03K

Interleukin 6 (IL-6, B cell Stimulatory Factor 2, BSF2, Cytotoxic T cell Differentiation Factor, CTL Differentiation Factor, CDF, Hepatocyte Stimulating Factor, HSF, Hybridoma Growth Factor, HGF, Hybridoma Plasmacytoma Growth Factor, HPGF, Interferon beta 2, IFNB2) (Azide Free)

Size

£819.00

SKU:: I8428-03K
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to chicken IL-6.
Format:: Supplied as a liquid in PBS. Azide free.
Extra Details:: Mammalian IL-6 is a multifunctional pro-inflammatory cytokine, which plays a major role in the regulation of immune responses, haematopoiesis and the induction of acute phase responses. The action of IL-6 occurs via the signal transducing receptor chain gp130 and the IL-6 receptor alpha chain is responsible for ligand binding. IL-6 is produced by a wide variety of cells including B lymphocytes, T lymphocytes, monocytes/macrophages, fibroblasts and endothelial cells and has a widespread cellular effect, including B and T lymphocytes, hepatocytes and cells of the CNS. Studies relating to the properties of chIL-6 have been limited, but demonstrate that hepatocyte-stimulating factor (HSF), secreted by lipopolysaccharide (LPS)-stimulated macrophages, induces fibronectin production in cultured chicken hepatocytes, and that HSF produced by LPS-treated fibroblasts, was neutralized by an antiserum to recombinant HuIL-6 (rHuIL-6). rHuIL-6 has also been shown as biologically active on chicken hepatocytes. Applications: Suitable for use in ELISA, Functional Assays, Western Blot and Neutralization. Other applications not tested. Recommended Dilutions: Neutralization: Neutralizes chicken IL-6 in 7D1 assays Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice