I8428-03B1

Interleukin 6 (IL-6, B cell Stimulatory Factor 2, BSF2, Cytotoxic T cell Differentiation Factor, CTL Differentiation Factor, CDF, Hepatocyte Stimulating Factor, HSF, Hybridoma Growth Factor, HGF, Hybridoma Plasmacytoma Growth Factor, HPGF, Interferon beta 2, IFNB2) (Biotin)

Size

£639.00

SKU:: I8428-03B1
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to human IL-6, expressed in E. coli.
Format:: Supplied as a lyophilized powder from PBS. No preservative added. Labeled with Biotin. Reconstitute with 500ul sterile PBS, 0.1% BSA.
Extra Details:: Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 0.25-1ug/ml, in conjunction with I8428-25A as a capture antibody, allows the detection of at least 0.2-0.4ng/well of recombinant human IL-6. Western Blot: 0.1-0.2ug/ml, the detection limit for recombinant Human IL-6 is 1.5-3ng/lane, under either reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS, 0.1% BSA.. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.