I8427-15C

Interleukin 5 (IL-5, BCDF-alpha, Eosinophil Differentiation Factor, EDF)

Size

£908.00

SKU:: I8427-15C
Purity:: Purified by Protein A affinity chromatography from ascites.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Purified natural mouse IL-5.
Clone:: 6A422 (TRFK5)
Format:: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Extra Details:: IL-5 is a T cell derived haematopoietic growth factor, which stimulates eosinophil differentiation and activation. This clone may be used to neutralise IL-5 bioactivity in a functional assay (1,2). Removal of sodium azide is recommended prior to use in functional assays. Applications: Suitable for use in Flow Cytometry, ELISA and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: Membrane permeabilization is required for this application. Optimal dilution determined by the researcher. Flow Cytometry: Recommended Secondary Reagents: F(ab')2 Goat anti rat IgG:FITC (mouse adsorbed) F(ab')2 Goat anti rat IgG:RPE (mouse adsorbed) Western Blot: Recommended Secondary Reagents: Goat anti rat IgG:HRP (mouse adsorbed) Recommended Negative Controls: Rat IgG1 Negative Control Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.