Interleukin 5 (IL-5, BCDF-alpha, B Cell Differentiation Factor I, Colony Stimulating Factor Eosinophil, Eosinophil Differentiation Factor, EDF, T Cell Replacing Factor, TRF) (Biotin)

Size

£657.00

SKU:: I8427-10F1
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to human IL-5, expressed in E. coli.
Format:: Supplied as a lyophilized powder from PBS. No preservative added. Labeled with Biotin. Reconstitute with 500ul sterile PBS, 0.1% BSA.
Extra Details:: Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 0.25-1ug/ml (using 100ul/well) Western Blot: 0.1-0.2ug/ml When used in conjunction with compatible secondary reagents, the detection limit for recombinant human IL-5 is 1.5 - 3ng/lane, under either reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.