I8427-06K
Interleukin 5 (IL-5, BCDF-alpha, Eosinophil Differentiation Factor, EDF) (Biotin)
Size
£466.00
- SKU:
- I8427-06K
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- COS-expressed recombinant human IL-5
- Clone:
- JES1-39D10
- Format:
- Supplied as a liquid in PBS, 0.09% sodium azide. Labeled with Biotin.
- Extra Details:
- Applications: ELISA: Monoclonal antibody is useful as a capture or detection antibody in a sandwich ELISA for quantifying human IL-5 protein levels.1-3,5 Purified antibody may be paired with biotinylated antibody as the detection antibody, with purified recombinant human IL-5 as the standard. Or, conversely, biotinylated may be paired with purified antibody as the capture antibody. Immunofluorescence/Flow Cytometry: This antibody is useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-5-positive cells within mixed cell populations.5,6 Recombinant human IL-5, unlabeled antibody, or Rat IgG1 should be used as a specificity control. Western Blotting: The purified antibody is also useful for Western blotting applications. A concentration of 1-5ug/ml (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of ≤ 100ng/lane of recombinant human IL-5 under reducing conditions. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice