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I8427-06J

Interleukin 5 (IL-5, BCDF-alpha, Eosinophil Differentiation Factor, EDF)

Size

£403.00

SKU:
I8427-06J
Purity:
Purified
Storage Conditions:
4°C (-20°C Glycerol)
Supplier:
United States Biological
Host:
Rat
Immunogen:
COS-expressed recombinant human IL-5
Clone:
5K121
Format:
Supplied as a liquid in 100mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added.
Extra Details:
Applications: Suitable for use in Immunofluorescence, Flow Cytometry, ELISA and Western Blot. Other applications not tested. Recommended Dilution: Immunofluorescence/Flow Cytometry: For intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-5-positive cells within mixed cell populations. Western Blot: 1-5ug/ml (≤100ng/lane) Detects recombinant human IL-5 under reducing conditions. Optimal dilutions to be determined by the researcher. Matched Pair: Capture: I8427-06J Interleukin 5 (IL-5, BCDF-alpha, Eosinophil Differentiation Factor, EDF) Dectection: I8427-06A Interleukin 5 (IL-5, BCDF-alpha, Eosinophil Differentiation Factor, EDF) (Biotin) Recommended Secondary: Western Blot: I1904-47J IgG, H&L (AP) GtxRt I1904-47P IgG, H&L, X-Adsorbed (AP) GtxRt I1904-47U IgG, H&L (AP) GtxRt Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Shipping Conditions:
Blue Ice