I8427-03E
Interleukin 5 (IL-5, BCDF-alpha, Eosinophil Differentiation Factor, EDF)
Size
£1,023.00
- SKU:
- I8427-03E
- Purity:
- Purified by Protein G affinity chromatography from ascites.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Ile20-Ser134 from human IL-5, expressed Sf21 cells.
- Clone:
- 8H215
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
- Extra Details:
- Interleukin 5 (IL-5) is a disulfide-linked homodimeric cytokine that is secreted by T cells. IL-5 promotes the proliferation, differentation and activation of eosinophils. It binds to a receptor complex consisting of one IL-5 specific alpha chain and one non-binding common beta chain that is shared with the receptors for GM-CSF and IL-3. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, and Flow Cytometry. Other applications have not been tested. Recommended Dilutions: Western Blot: 1ug/ml using recombinant human IL‑5 Immunocytochemistry: 8-25ug/ml using man peripheral blood lymphocytes Flow Cytometry: 2.5ug/ml labels 10e6 cells using Ca2+ionomycin-treated human PBMCs Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-65V IgG, F(ab’)2 (FITC) Pab RbxMo I1904-66A IgG, F(ab’)2, X-Adsorbed (FITC) ) Pab RbxMo Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice