Interleukin 4 Receptor alpha (IL-4Ra) (PE)

Size

£754.00

SKU:: I8426-70B
Application:: Flow Cytometry
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: 4°C Do not freeze
Supplier:: United States Biological
Host:: Goat
Immunogen:: Recombinant protein corresponding to mouse Interleukin 4 Receptor alpha extracellular domain expressed in NSO cells.
Format:: Supplied as a liquid in PBS, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
Extra Details:: Interleukin 4 is a pleiotropic cytokine produced primarily by activated T cells, mast cells and basophils. The diverse biological effects of IL-4 on a variety of cell types are mediated by the binding of IL-4 to specific cell surface receptors. As is the case with many other cytokines, the functional high-affinity receptor for IL-4 is a complex consisting of a ligand binding subunit and a second subunit (Beta chain) that can modulate the ligand binding affinity of the receptor complex. It has been shown that in certain cell types, the gamma chain of the IL-2 receptor is a functional component (Beta chain) of the IL-4 receptor complex. cDNA clones for the ligand binding chain (IL-4 R) of both the mouse and human high affinity IL-4 receptors have been isolated. The human or mouse IL-4 R is an approximately 140kD transmembrane protein containing an extracellular domain, a transmembrane domain, and a large cytoplasmic domain that is essential for IL-4 signal transduction. In addition to the cDNA clone encoding the full-length transmembrane protein, a second cDNA clone that arises from alternate splicing encodes a soluble secreted form of IL-4 R that has been isolated from mouse cells, but not yet from human sources. A naturally-occurring soluble form of the IL-4 R has also been identified in mouse biological fluids and in murine cell culture supernatants. Native or recombinant murine soluble IL-4 R, as well as recombinant human soluble IL-4 R, can bind IL-4 with the same affinity as the membrane bound IL-4 R. Soluble IL-4 R is a competitive inhibitor of IL-4 and has been shown to effectively neutralize many IL-4-mediated responses both in vivo and in vitro. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat. Use 10ul to label 10e6 cells. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Shipping Conditions:: Blue Ice