I7663-97C
Interleukin 33 (IL-33)
Size
£863.00
- SKU:
- I7663-97C
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Recombinant protein corresponding to Ser112-Thr270 from human IL-33, expressed in E. coli.
- Clone:
- 7H186
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Interleukin-33 (IL-33), also known as NF-HEV and DVS 27, is a member of IL-1 cytokine family. IL-33 is constitutively expressed in smooth muscle and airway epithelia and is upregulated by IL-1 Alpha or Beta in arterial smooth muscle, dermal fibroblasts, and keratinocytes. IL-33 is synthesized as a 270aa, 32kD proform that localizes to the nucleus and may regulate gene transcription. Cleavage generates mature IL-33 as an 18kD C-terminal proinflammatory fragment which binds IL-1 R4/ST2 on Th2 and mast cells. Mature human IL-33 shares 55% aa identity with mouse IL-33. Applications: Suitable for use in Western Blot, Immunocytochemistry and ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml using recombinant human IL33 Immunocytochemistry: 8-25ug/ml using immersion fixed human peripheral blood mononuclear cells treated with PMA and Ca2+ ionomycin Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice