I7663-97B
Interleukin 33 (IL-33)
Size
£889.00
- SKU:
- I7663-97B
- Purity:
- Purified by Immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Recombinant protein corresponding to Ser109-Ile266 from mouse IL-33 expressed in E. coli.
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- IL-33 is a member of the IL-1 cytokine family and is constitutively expressed in smooth muscle cells and the airway epithelium. IL-33 signals through receptors, IL-1RL1 and IL-1RAP, to induce type 2 cytokine production in polarized Th2 cells. Applications: Suitable for use in Western Blot, Flow Cytometry, Immunocytochemistry, ELISA, Immunohistochemistry and Neutralization. Other applications not tested. Recommended Dilutions: Western Blot: 0.4ug/ml using HEK293T human embryonic kidney cell line transfected with full length mouse IL-33. Flow Cytometry (Intracellular): 2.5ug/10e6 mouse splenocytes treated with PMA and Ca2+ ionomycin, fixed with paraformaldehyde and permeabilized with saponin. Immunocytochemistry: 5-15ug/ml using Immersion fixed bEnd.3 mouse endothelioma cell line Immunohistochemistry: 5-15ug/ml using perfusion fixed frozen sections of mouse spleen ELISA (Capture): 0.2-0.8ug/ml Neutralization: 10-50ng/ml in the presence of 0.25ng/ml of recombinant mouse IL-33. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice