Interleukin 33 (IL-33)

Size

£978.00

SKU:: I7663-97A
Purity:: Purified by immunoaffinity chromatography
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: E. coli-derived recombinant human IL-33 (aa112-270)
Format:: Supplied as a powder lyophilized from a from a 0.2um filtered solution in PBS containing trehalose. Reconstitute to 0.2mg/ml with 500ul sterile PBS.
Extra Details:: Applications: Suitable for use in ELISA, Immunocytochemistry, Neutralization and Western Blot. Other applications have not been tested. Recommended Dilution: ELISA: 0.2-0.8ug/ml as capture antibody Immunocytochemistry: 5-15ug/ml using immersion fixed human PBMCs treated with PMA and calcium ionomycin Neutralization: Measured by its ability to neutralize IL33induced proliferation in the D10.G4.1 mouse helper T cell line. The Neutralization Dose (ND50) is typically 0.753.0ug/ml in the presence of 1 ng/mL recombinant human IL33 and suboptimal amounts of mouse CD3-epsilon monoclonal antibody. Western Blot: 0.1ug/ml using rhIL-33 as sample Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute with sterile PBS. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.