I7663-39L

Interleukin 3 (IL-3)

Size

£403.00

SKU:: I7663-39L
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Yeast-expressed recombinant human IL-3
Clone:: BVD8-3G11
Format:: Supplied as a liquid in 100mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added.
Extra Details:: Applications: ELISA Capture: Purified monoclonal antibody is the preferred capture antibody in a sandwich ELISA for quantifying human IL-3 protein levels.1-3 The purified antibody should be paired with biotinylated antibody as the detection antibody (e.g., I7663-39P) with purified recombinant human IL-3 as the standard. Western Blotting: The purified antibody is also useful for Western blotting applications. A concentration of 1-5ug/ml (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of ≤ 100ng/lane of recombinant human IL-3 under reducing conditions. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.