I7663-31L-ML550

Interleukin 2 Receptor, alpha (IL-2Ra, IL-2R alpha, CD25, IDDM10, Ly43, p55, T Cell Growth Factor Receptor, TCGFR, TAC Antigen) (MaxLight 550)

Size

£856.00

SKU:: I7663-31L-ML550
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Bovine Interleukin 2 Receptor, alpha.
Clone:: 10B1132 (IL-A111)
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.
Extra Details:: MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation and Functional Assays. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50-100; 10ul labels 10e6 cells in 100ul Immunohistochemistry: Frozen sections Functional Assays: Removal of sodium azide is required. Optimal dilutions to be determined by the researcher. Hybridoma: 63-Ag8.653 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Applications are based on unconjugated antibody.