I7663-27N4
INTERLEUKIN-2 (FITC) (IL-2)
Size
£620.00
- SKU:
- I7663-27N4
- Purity:
- Purified by Protein G affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Clone:
- MQ1-17H12
- Format:
- Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.09% sodium azide. Labeled with Fluorescein Isothiocyanate Isomer 1 (FITC).
- Extra Details:
- IL-2 is a 153 amino acid secreted cytokine produced by T-cells following antigenic or mitogenic stimulation. It plays a crucial role in T-cell proliferation and immune regulation. Interleukin-2, clone 3H2514 has been sucessfully used for the detection and measurement of native and recombinant human IL-2 using flow cytometry and ELISA where it acts as the capture reagent in a very sensitive assay for IL-2 determination in human samples. IL-2 is used extensively for the treatment of patients with metastatic melanoma (Atkins et al. 1999) and renal cancer with reproducible benificial effects (Rosenberg 2014). Monitoring of IL-2 levels in such patients is required for optimization of treatment regimens (Lotze et al. 1986). This antibody is also useful for evaluating levels of IL-2 in patients with autoimmune conditions including lupus erythematosus and rheumatoid arthritis where there may be an impaired ability to produce IL-2 (Talal et al. 1983). Applications: Suitable for use in Flow Cytometry and ELISA. Other applications not tested. Recommended Dilution: Flow Cytometry: Neat, Use 10ul to label 10e6 cells in 100ul. Membrane permeabilization is required. Optimal dilution determined by the researcher Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice