Interleukin 1 beta (IL1b, IL-1 beta, Catabolin, Hematopoietin 1, H1, IFN beta Inducing Factor, IL1F2, LAF, OAF, Osteoclast Activating Factor, Preinterleukin beta, Pro Interleukin 1 beta)

Size

£416.00

SKU:: I7663-24B
Purity:: Purified by immunoaffinity chromatography
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant mouse Interleukin-1 beta (IL-1b), expressed in E. coli
Format:: Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile ddH2O to 0.1-1mg/ml. Do not vortex.
Extra Details:: Applications: Suitable for use in Neutralization, Sandwich ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Sandwich ELISA: 0.5-2.0ug/ml using 100ul/well,. The detection is ~2000-4000pg/ml of recombinant mouse Interleukin 1 beta (IL1b). Western Blot: 0.1-0.2ug/ml. Detects 1.5-3ng/lane, under either reducing or non-reducing conditions. Neutralization: 0.57-0.86ug/ml Yields one-half maximal inhibition [ND50] of the biological activity (10pg/ml)). Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Country of Origin: USA