I7663-11K

Interleukin 1 beta (IL-1b)

Size

£332.00

SKU:: I7663-11K
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to sheep IL-1 Beta
Clone:: 3H2506 (1D4)
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Extra Details:: Interleukin-1 beta (IL-1beta) is a potent immunomodulator which mediates a wide range of immune and inflammatory responses, including the activation of B and T cells. Mouse IL-1b is a 17.5kD protein containing 153aa residues. Applications: Suitable for use in ELISA, Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:10. Use 10ul to label 10e6 cells in 100ul. Membrane permeabilization is required. Immunohistochemistry: Frozen sections Optimal dilutions to be determined by researcher. Recommended Secondary Antibodies: I1904-65W: IgG, F(ab’)2 (HRP) Pab Rb xMo I1904-66A: IgG, F(ab’)2 (FITC) Pab Rb xMo Recommended Negative Control: I1904-78R: IgG1 Murine Negative Control Recommended Pair (only for sandwich detection of bovine IL-1b): Capture: I7663-11K Detection: I7663-10E Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.