I7663-01
Interleukin 1 alpha, Recombinant, Mouse (IL-1a, Hematopoietin-1, Lymphocyte-activating Factor, LAF, Endogenous Pyrogen, Leukocyte Endogenous Mediator, LEM, Mononuclear Cell Factor, MCF)
Size
£512.00
- SKU:
- I7663-01
- Molecular Weight:
- 17.9
- Purity:
- ≥98% (HPLC, SDS-PAGE). Endotoxin: ≤0.1ng/ug
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Format:
- Supplied as a lyophilized powder from PBS. Reconstitute with sterile ddH2O to ≥0.1mg/ml.
- Extra Details:
- IL-1a is a non-secreted, proinflammatory cytokine produced in a variety of cells, including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1a and IL-1b bind to the same receptor and have similar, if not identical, biological properties. These cytokines have a broad range of activities including the stimulation of thymocyte proliferation by inducing IL-2 release, B cell maturation and proliferation, mitogenic FGF-like activity, and the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1b is a secreted cytokine, IL-1a is predominantly a cell-associated cytokine. Recombinant mouse IL-1a is a 17.9kD protein containing 156 amino acid residues. Amino Acid Sequence: SAPYTYQSDL RYKLMKLVRQ KFVMNDSLNQ TIYQDVDKHY LSTTWLNDLQ QEVKFDMYAY SSGGDDSKYP VTLKISDSQL FVSAQGEDQP VLLKELPETP KLITGSETDL IFFWKSINSK NYFTSAAYPE LFIATKEQSR VHLARGLPSM TDFQIS Biological Activity: The ED50 as determined by the dose-dependent stimulation of murine D10S cells is ≤0.002ng/ml, corresponding to a specific activity of ≥5x10e8 units/mg. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice