I7662-88A
Interferon Regulatory Factor 3 (IRF3)
Size
£850.00
- SKU:
- I7662-88A
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Recombinant protein corresponding to Val206-Ser427 from human IRF3, expressed in E. coli. Species Sequence Homology: mouse; 76% and porcine; 83%
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- Interferon regulatory factor 3 (IRF3) is a 50 kDa, 427aa, member of the IRF family of proteins. Human IRF3 is a transcription factor that binds the interferon-sensitive response element (ISRE) and is activated by Toll-like receptors, TLR3 and TLR4. Viral entry or engagement of TLR3 or TLR4 stimulates IRF3 phosphorylation, nuclear translocation and stimulation of IFN production. Applications: Suitable for use in Western Blot, Flow Cytometry, Immunocytochemistry and ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml in lysates of HeLa human cervical epithelial carcinoma cell line, U937 human histiocytic lymphoma cell line, Raji human Burkitt's lymphoma cell line and Daudi human Burkitt's lymphoma cell line Flow Cytometry (Intracellular): 2.5ug/10e6 cells using Daudi human Burkitt’s lymphoma cell line fixed with paraformaldehyde and permeabilized with saponin Immunocytochemistry: 5-15ug/ml using immersion fixed HeLa human cervical epithelial carcinoma cell line. Optimal dilutions to be determined by researcher.. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice