I7662-17Q2

Interferon gamma (IFN-gamma)

Size

£1,004.00

SKU:: I7662-17Q2
Purity:: Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Recombinant protein corresponding to Gln24-Thr166 from bovine IFN-gamma, expressed in E. coli.
Clone:: 9H136
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
Extra Details:: Mature Interferon gamma (IFN-gamma) is a homodimeric cytokine produced primarily by T-lymphocytes and natural killer cells. IFN-gamma has many immunoregulatory and proinflammatory activities. It binds to IFN-gamma receptors present on virtually all cell types. The 17kD mature bovine IFN-gamma shares 97% aa sequence identity with ovine IFN-gamma and 77%-80% with canine, equine, and porcine IFN-gamma. It shares 61%, 40%, and 42% identity with human, mouse, and rat IFN-gamma, respectively. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: ELISA: 2-8ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.