Interferon gamma Receptor Chain 1, neutralizing (CDw119, IFNgR1)

Size

£876.00

SKU:: I7662-17K1
Purity:: Purified by immunoaffinity chromatography. Endotoxin: ≤0.1EU/1ug by LAL.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Recombinant corresponding to Glu18-Gly245 of Interferon gamma Receptor Chain 1(G) expressed in NSO cells (P15260).
Format:: Supplied as a liquid in PBS, pH 7.4, 5% trehalose. No preservative added.
Extra Details:: IFNg induces a variety of biological responses, such as antiviral, antiproliferative and immunomodulatory activity in sensitive cells. Activation of the IFNg receptor (IFNgR) leads to auto- phosphorylation of the Janus kinases JAK1 and JAK2, and the nuclear translocation of the transcription factors Stat1a p91 and Stat1b p84. The IFNgR is composed of at least two chains, designated IFNgR1 and IFNgR2. Although expression of IFNgR1 is sufficient for ligand binding, it alone does not confer responsiveness to IFNg. Concomitant expression of IFNgR1 and IFNgR2 is required for transcriptional activation of IFNg- inducible genes. Applications: Suitable for use in ELISA, Western Blot, Immunocytochemistry and Neutralization. Other applications not tested. Recommended Dilutions: Neutralization: 0.5-2ug/ml using ~2ng/ml of Recombinant human Interferon gamma. Measured by it’s ability to neutralize IFN-gamma r1/cd119-mediated inhibition of EMCV-induced cytopathy in the HeLa human cervical epithelial carcinoma cells line. Western Blot: 0.1ug/ml Immunocytochemistry: 5-15ug/ml. Immersion fixed human peripheral blood mononuclear cells. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice