I7662-02A
Interferon alpha (IFNa)
Size
£745.00
- SKU:
- I7662-02A
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Clone:
- 6A565 (F18)
- Format:
- Supplied as a liquid in PBS, 0.1% BSA.
- Extra Details:
- Monoclonal antibodies directed against alpha Interferon make it possible to interpret results obtained from crude materials containing both alpha and beta Interferon. The difficulties in studying in vitro and in vivo effects of "type 1" Interferons arise from the fact that both alpha and beta Interferons are produced in response to the same stimuli and also seem to act via the same receptor. These interferon activities can only be distinguished from one another by use of specific neutralizing antibodies. Applications: Suitable for use in ELISA, Flow Cytometry, Western Blot and Neutralization. Other applications have not been tested. Recommended Dilutions: Flow Cytometry: 1:50. For intracellular staining of IFN-alpha, cells can be fixed in 1% formaldehyde; blocked and permeabilized in 0.2% saponin, 5% normal rabbit serum for 30 minutes on ice. Neutralization of Biological Activity: 1ug per mouse i.v. before treatment with 35ug LPS i.p., decreased the LPS-induced IL-1beta response. Optimal dilutions to be determined by the researcher. Recommended Positive Control: Plasmacytoid dendritic cells Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice