I7661-68J5
Interferon gamma (IFN-gamma, IFNg, BoIFNG) (PE)
Size
£1,055.00
- SKU:
- I7661-68J5
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Bovine Interferon gamma. Species Sequence Homology: Mustelid.
- Clone:
- CC302 (10D305)
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 1% BSA, 0.09% sodium azide, 5% sucrose. Labeled with R-Phycoerythrin (PE). Reconstitute with 1ml sterile ddH2O.
- Extra Details:
- Interferon-gamma (IFN-gamma) is a lymphoid factor which possesses potent anti-viral activity. It has also been shown to stimulate macrophages and NK cells. Rat IFN-gamma is a 15.635kD protein. Interferon gamma is the only known type II interferon. Under physiological conditions, interferon gamma is induced when T-lymphocytes, previously sensitized to an antigen, are exposed to the same antigen, hence its other name: immune interferon. Interferon gamma has demonstrated significant anti-proliferative effects in in vivo studies; however, its therapeutic effects have been limited. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: Neat-1:10; 10ul labels 10e6 cells in 100ul. Membrane permeabilization is required for this application. Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at 4°C. Reconstitute with sterile ddH2O. DO NOT FREEZE! For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity.
- Shipping Conditions:
- Blue Ice